Actividad antibacteriana del aceite esencial de orégano mexicano (Lippia graveolens H.B.K.)
DOI:
https://doi.org/10.59741/agraria.v5i1-2-3.424Keywords:
Bacteria, method of dilution, hidrodestilation, carvacrol, p-cymenoAbstract
The antibacterial activity, and components of the essential oil obtained by hidrodestilation of the aerial parts (leaves and flowers) of Lippia graveolens H.B.K. plants, collected in Torreón, Coah., Mexico, were as sayed. The analysis of the main constituent members (CP) was performed by means of nuclear magnetic resonance (R M N) of ¹³C, and antibacterial activity. Determining the minimal inhibitory concentration was performed by the method of broth dilution, using Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853 strains, and two isolated strains of cow milk with mastitis: Escherichia coli and Staphylococcus aureus. The main obtained constituent members were: car vacrol (72 %) and its precursor, p-cymeno (28 %). Both percentages were high in relation to the reported ones in other assays (0,02 to 44,8 % for carvacrol and 6,8 to 21,8 % for p-cymeno). As compared with the ATCC strains, E. coli and S. aureus isolated of milk with mastitis were more sensitive to the oregano oil (10 vs µL mL-1 1 in E. coli and 5 vs 1.25 in S. aureus) while Ps. aureuginosa required 2,5 µL mL-1. The antibacterial activity shown by the oregano essential oil was effective against the pathogenic bacteria. This demonstrates its potential use as a therapeutic agent.
Downloads
References
Bagamboula, C., M. Uyttendaele y J. Debevere. 2004. Inhibitory effect of thyme and basil essential oils, car vacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiol. 21: 33-42. DOI: https://doi.org/10.1016/S0740-0020(03)00046-7
Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods: a review. Int. J. Food Microbiol. 94(3): 223-53. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
Cowan, M. M. 1999. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12(4): 564-82. Dorman, H. J. y S. G. Deans 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J.Appl. Microbiol. 88(2): 308-16. DOI: https://doi.org/10.1128/CMR.12.4.564
Gulluce, M., M. Sokmen, D. Daferera, G. Agar, H. Ozkan, N. Kartal, M. Polissiou, A. Sokmen y F. Sahin 2003. In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. J. Agric. Food Chem. 51(14): 3958-65. DOI: https://doi.org/10.1021/jf0340308
Hammer, K. A., C. F. Carson y T. V. Riley 1998. In vitro activity of essential oils, in particular Melaleuca alternifolia (tea tree) oil and tea tree oil products, against Candida spp. J. Antimicrob. Chemother. 42(5): 591-5. DOI: https://doi.org/10.1093/jac/42.5.591
Kalemba, D. y A. Kunicka 2003.Antibacterial and anti fungal properties of essential oils. Curr Med. Chem. 10(10): 813-29. DOI: https://doi.org/10.2174/0929867033457719
Nelson, R. R. S. 2003. In-vitro activities of five plant es sential oils against methicillin-resistant Staphylococ cus aureus and vancomycin-resistant Enterococcus faecium. J. Antimicrob. Chemother. 40: 305-6. DOI: https://doi.org/10.1093/jac/40.2.305
Ohno, T., M. Kita, Y. Yamaoka, S. Imamura, T. Yamamoto, S. Mitsufuji, T. Kodama, K. Kashima y J. Imanishi. 2003. Antimicrobial activity of essential oils against Helicobacter pylori. Helicobacter 8(3): 207-15. DOI: https://doi.org/10.1046/j.1523-5378.2003.00146.x
Pascual, M. E., K. Slowing, E. Carretero, D. Sanchez Mata y A. Villar. 2001. Lippia: traditional uses, chemistry and pharmacology: a review. J. Ethnopharmacol. 76(3): 201-14. DOI: https://doi.org/10.1016/S0378-8741(01)00234-3
Perez-Galindo, J. A., J. Lopez-Miranda y I. R. Martinez Dominguez. 2000. Geometric and Reynolds number efects on oregano (Lippia berlandieri Schauer) es sential oil extraction. J. Food Eng. 44: 127-33. DOI: https://doi.org/10.1016/S0260-8774(99)00154-5
Rastrelli, L., A. Caceres, C. Morales, F. De Simone y R. Aquino. 1998. Iridoids from Lippia graveolens. Phy tochemistry 49(6): 1829-1832. DOI: https://doi.org/10.1016/S0031-9422(98)00196-4
Salgueiro, L. R., C. Cavaleiro, M. J. Goncalves y A. P. da Cunha. 2003a.Antimicrobial activity and chemical com position of the essential oil of Lippia graveolens from Guatemala. Planta Medica 69(1): 80-83. DOI: https://doi.org/10.1055/s-2003-37032
Salgueiro, L. R., C. Cavaleiro, E. Pinto, C. Pina-Vaz, A. G. Rodrigues, A. Palmeira, C. Tavares, S. Costa-de Oliveira, M. J. Goncalves y J. Martinez-de-Oliveira. 2003b. Chemical composition and antifungal activity of the essential oil of Origanum virens on Candida spe cies. Planta Med 69(9): 871-4.
Schwäm mLe, B., E. Winkelhausen, S. Kuzmanova y W. Steiner. 2001. Isolation of carvacrol assimilating mi croorganisms. Food Technol. Biotechnol. 39(4): 341- 345.
Stashenko, E. E., B. E. Jaramillo y J. R. Martínez. 2004. Analysis of volatile secondary metabolites from colombian Xylopia aromatica (Lamarck) by different extraction and headspace methods and gas chroma tography. J. Chromatogr. A 1025(1): 105-13. DOI: https://doi.org/10.1016/j.chroma.2003.10.059
Ultee, A., M. H. Bennik y R. Moezelaar. 2002. The phe nolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus.Appl. Environ. Microbiol. 68(4): 1561-8. DOI: https://doi.org/10.1128/AEM.68.4.1561-1568.2002
Ultee, A., E. P. Kets y E. J. Smid 1999. Mechanisms of action of carvacrol on the food-borne pathogen Bacil lus cereus. Appl. Environ. Microbiol. 65(10): 4606- 4610. DOI: https://doi.org/10.1128/AEM.65.10.4606-4610.1999
Valentao, P., E. Fernades, F. Carvalho, P. B. Andrade, R. M. Seabra y M. L. Bastos. 2002. Studies on the Anti oxidant Activity of Lippia citriodora Infusion: Scav enging Effect on Superoxide Radical, Hydroxyl Radi cal and HypochlorousAcid. Biol. Pharm. Bull. 25(10): 1324-1327. DOI: https://doi.org/10.1248/bpb.25.1324
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
PLUMX Metrics
