Resistencia de plantas de maíz a la infección por Aspergillus Flavus Link en invernadero
DOI:
https://doi.org/10.59741/agraria.v8i2.456Keywords:
Inoculation technique, infected grain, mycotoxinsAbstract
Corn Plant Resistance to Infection by Aspergillus flavus Link in Greenhouse conditions. Production of aflatoxins and percentage of infected grain in four genotypes of maize: ZMT1, ZMT2, ZMS1, ZMS2 were evaluated and inoculated with Aspergillus flavus L. under greenhouse conditions, taking as reference materials genotypes H-443A and DK2020Y, reported to be, respectively, resistant and susceptible to accumulation of aflatoxin in the field. Styles (silk) of forming ears were sprayed with a suspension of 7 x 107 A. flavus L. spores. The infection was observed as a localized rot at the top of the ears. The percentage of grain infected with the pathogen, as well as the concentration of aflatoxins were determined for each variety. The H-443A genotype remained free of infection and aflatoxins, in contrast to the genotype DK2020Y, in which ear rot in 40% of infected plants, and 245 ppb of aflatoxin in grain were observed. ZMT1, ZMT2, ZMS2, and ZMS1 genotypes had similar percentages of plants with ear rot and infected grain. ZMT2 accumulated the highest amount of aflatoxin (510 ppb), and in ZMS1 were not detected.
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Association of Analytical Communities (AOAC). 1995. Official Method 991.31 Aflatoxins in corn, raw pea nuts and peanut butter: Immunoaffinity Column (Aflatest) method. First Action 1991. AOAC-IUPAC Method. pp. 20-21. In: AOAC International (Ed.) Of ficial Method of analysis. Natural Toxins. Chapter 49. Arlington, VA, USA. 325 p.
Benítez-Esquivel, G. 2007. Identificación de genes de resistencia a sequía y su relación con la contaminación con aflatoxinas en maíz. Tesis de Maestría en Ciencias. UNAM. Facultad de Estudios Superiores- Cuautitlán. Cuautitlán Izcalli, Edo. Mex., México. 81 p.
Bogantes-Ledezma, P., D. Bogantes-Ledezma y S. Bogantes-Ledezma. 2004. Aflatoxinas. Acta Méd. Costarric. 46 (4): 174-178. DOI: https://doi.org/10.51481/amc.v46i4.157
Brooks, T.D., W.P. Williams, G.L. Windham, M.C Willcox and H.K. Abbas. 2005. Quantitative trait loci contrib uting resistance to aflatoxin accumulation in the maize inbred Mp313E. Crop Sci. 45: 171–174. DOI: https://doi.org/10.2135/cropsci2005.0171a
Bucio-Villalobos, C.M., D. Guzmán-de-Peña y J.J. Peña Cabriales. 2001. Aflatoxin synthesis in corn fields in Guanajuato, Mexico. Rev. Iberoam. Micol. 18: 83- 87.
Busboom, K.N. and D.G. White. 2004. Inheritance of re sistance to aflatoxin production and Aspergillus ear rot of corn from the cross of inbreds B73 and Oh516. Phytopathol. 94: 1107-1115. DOI: https://doi.org/10.1094/PHYTO.2004.94.10.1107
Cobielles-Castrejón, G. 2007. Identificación de genes relacionados a vigor, longevidad y contaminación con aflatoxinas en semillas de maíz. Tesis de Maestría en
Ciencias. UNAM. Facultad de Estudios Superiores Cuautitlán. Cuautitlán Izcalli, Edo. Mex. México. 61 p. García-Aguirre, G., R. Martínez-Flores y J. Melgarejo Hernández. 2001. Inspección para aflatoxinas en el maíz almacenado o transportado en el estado de Sonora. 1998. Ann. Inst. Biol. UNAM S. Bot. 72(2): 187-193.
García-Aguirre, G., R. Martínez-Flores y J. Melgarejo Hernández. 2003. Inspecciones para determinar aflatoxinas en maíz en México: I Sonora. In: I Panamerican Symposium on Mycotoxins for Industry. April 1-4, 2003. Abstract in Proceedings book. Latinoamerican Society of Mycotoxicology. Mexico, D.F. p. 58.
Guo, B., Z.Y. Chen, R.D. Lee and B.T. Scully. 2008. Drought stress and preharvest aflatoxin contamination in agricultural commodity: genetics, genomics and proteomics. J. Integr. Plant Biol. 50 (10): 1281-1291. DOI: https://doi.org/10.1111/j.1744-7909.2008.00739.x
Martínez-Flores, R., G. García-Aguirre y J. Melgarejo Hernández. 2003a. Inspecciones para determinar aflatoxinas en maíz en México: II Tamaulipas. In: I Panamerican Symposium on Mycotoxins for Industry. April 1-4, 2003. Abstract in Proceedings book. Latinoamerican Society of Mycotoxicology. Mexico, D.F. p. 59.
Martínez-Flores, R., G. García-Aguirre y J. Melgarejo Hernández. 2003b. Inspección de aflatoxinas en maíz cultivado, almacenado y transportado en el estado de Tamaulipas, México, en 1998. Ann. Inst. Biol. UNAM S. Bot. 74 (2): 313-321.
Moreno-Lara, J. 2004. Estudio comparativo de Aspergil lus flavus y Aspergillus parasiticus en la producción de aflatoxinas bajo diferentes condiciones de humedad y temperatura. Tesis de Maestría en Ciencias. Universidad Nacional Autónoma de México. Facultad de Estudios Superiores- Cuautitlán. Cuautitlán Izcalli, Edo. Mex., México. 146 p.
O’brian, G.R., D.R. Georgianna, J.R. Wilkinson, J. Yu, H.K. Abbas, D. Bhatnagar, W. Nierman and G.A. Payne. 2007. The effect of elevated temperature on gene transcription and aflatoxina biosíntesis. Mycol. 99 (2): 232-239. DOI: https://doi.org/10.1080/15572536.2007.11832583
Plasencia, F.J. 2004. Aflatoxins in Maize: A Mexican Per spective. J. Toxicol. 23 (2 & 3): 155-177. DOI: https://doi.org/10.1081/TXR-200027809
Reyes, C., M. Cantú, I. Garza y H. Córdova. 2008. H 443A: Primer híbrido de maíz comercial en México tolerante a aflatoxinas. Memorias del XXII Congreso Nacional y Segundo Internacional de Fitogenética, 21 al 26 de septiembre de 2008, Chapingo Edo. Mex., México. No. de trabajo: 244.
Scheidegger, K.A. and G.A. Payne. 2003. Unlocking the secrets behind secondary metabolism: a review of As pergillus flavus from pathogenicity to functional genomics. J. Toxicol. 22: 423-429. DOI: https://doi.org/10.1081/TXR-120024100
Tubajika, K.M., H.J. Mascagni, K.E. Damann and J.S.
Russin. 2000. Aflatoxin production in corn by Aspergil lus flavus relative to inoculation, planting date, and harvest moisture in Louisiana. LAES Research Report Number 102 - March 2000.
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